The Genoa Organ Perfusion System is an ongoing open-source and low-cost project.

It is a  multipurpose device for organ perfusion developed with open-source microcontrollers, cheap materials, and reverse-engineering commercial sensors.

The basic perfusion system comprises a power supply, a control unit, two peristaltic pumps, pressure transducers, an oxygenator, and a thermostatic unit.

Power supply:  a AC/DC 12 Volt switching power supply

Peristaltic unit: it consists of two peristaltic pumps driven by stepper motors

Damper: it is a 100 ml container  half filled in order to reduce the flow fluctuations due to the peristaltic pump

Oxygenator: is a micro device based on hollow-fiber 

Pressure transducers: commercial disposable pressure transducers that are commonly used  in the operating room and in intensive care are used.

Control unit: it is composed of a microcontroller (Arduino Mega) which receives the pressure signals and controls the peristaltic pumps according to what is programmed.

Thermostatic unit: it consists of resistive elements controlled by a thermostat

Perfusion chamber: it is a Plexiglas container where the organ is placed during perfusion.

This is the best and fully functional version of the perfusion system.

For each part, I will outline the major mistakes I made, the solutions I discovered, and the problems I still face.